Rachael Ndege Virtual Hub 

Virtual Assistant

Yuta Media

Project Manager & Assistant

The Resume Revive Career Services

Equity Bank Limited

-Performed over 200 cash withdrawals and deposits for clients daily.
-Organized and filed processed checks and wire transfer documents for archiving.
-Marketed and onboarded clients on the usage of the different online banking platforms to make financial transactions more convenient.
-Directed clients and customers to respective officers for service.
-Answered customers' questions regarding banking policies.

Relationship Officer

Rachael Ndege

Biogen

Scientist I

AXONIS Therapeutics

Determine the mechanism of action of specific drugs in the enhancement of KCC2, a chloride transporter, which is downregulated after spinal cord injury.

Associate Scientist

Dana-Farber Cancer Institute

Post-doctoral fellow at Dana Farber Cancer Institute

	Executed experimental protocols involving the processing and culturing of mammalian cells
	Helped characterize the mechanism of action of the anti-oncogenic drugs pyrimethamine and fludrocortisone
	Determined the effect of different siRNAs against PABPC4 in the expression of STAT3 target genes
	Verified the viability of transfected cells (siRNA PABPC4) under pyrimethamine treatments using ATP-dependent assays
	Screened drugs targeting chromatin remodeling pathways using luciferase reporter cell lines

Post-doctoral fellow

University of Massachusetts Boston

	Identified plant enzymes involved in the degradation of polycyclic aromatic hydrocarbon (PAHs) using a combined genetic, biochemical and molecular approach
	Characterized gain-of-function and loss-of-function plant mutant lines in response to PAH-induced stress
	Trained and supervised ~25 undergraduate students running individual projects in the lab
	Taught practice laboratories of General Biology, Cell Biology, and Endocrinology
	Discussion of theory and procedure for the experiments
	Preparation of reagents for the practice laboratories
	Supervision of students 
	Grading tests and reports

Researcher/ Associate lecturer

Experienced biologist with a demonstrated history of working in the higher education industry and pharmaceutical companies. Skilled in Cell and Molecular Biology techniques. Strong professional background with a Doctor of Philosophy (PhD) focused in Biotechnology/ Molecular Biology from University of Massachusetts Boston. 

 I'm actively seeking new opportunities to apply my unique skill set. 

RESEARCH and TECHNICAL SKILLS
	Cell culture
	Cell-based assays (luciferase activity assays, viability assays)
	CRISPR-Cas9 genome editing technology
	Chromatin Immunoprecipitation (ChIP) Assay, Co-immunoprecipitation (co-IP)
	INTACT (isolation of nuclei tagged in specific cell types)
	Confocal microscopy and epifluorescence microscopy
	Immunohistochemistry, RIA, and ELISA
	SDS-PAGE and Western blotting
	Gene cloning, bacterial transformation, DNA/RNA extraction and purification
	Protein expression and purification (Nickel affinity chromatography, SEC, HIC)
	Gene expression analysis (qRT-PCR, PCR, Northern blot)


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Rachael Ndege

Juan C. Hernandez, Ph.D.

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