Juan C. Hernandez, Ph.D.'s Linkedin Analytics

Experienced biologist with a demonstrated history of working in the higher education industry and pharmaceutical companies. Skilled in Cell and Molecular Biology techniques. Strong professional background with a Doctor of Philosophy (PhD) focused in Biotechnology/ Molecular Biology from University of Massachusetts Boston. I'm actively seeking new opportunities to apply my unique skill set. RESEARCH and TECHNICAL SKILLS  Cell culture  Cell-based assays (luciferase activity assays, viability assays)  CRISPR-Cas9 genome editing technology  Chromatin Immunoprecipitation (ChIP) Assay, Co-immunoprecipitation (co-IP)  INTACT (isolation of nuclei tagged in specific cell types)  Confocal microscopy and epifluorescence microscopy  Immunohistochemistry, RIA, and ELISA  SDS-PAGE and Western blotting  Gene cloning, bacterial transformation, DNA/RNA extraction and purification  Protein expression and purification (Nickel affinity chromatography, SEC, HIC)  Gene expression analysis (qRT-PCR, PCR, Northern blot)